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  • Occurrence, characterizatio...
    Naas, Hesham T; Edarhoby, Ramadan A; Garbaj, Aboubaker M; Azwai, Salah M; Abolghait, Said K; Gammoudi, Fatim T; Moawad, Ashraf A; Barbieri, Ilaria; Eldaghayes, Ibrahim M

    Veterinary World, 06/2019, Letnik: 12, Številka: 6
    Journal Article

    The aim of the current investigation was to screen the presence of spp., especially in meat, meat products of different animal species, and some seafood sold in some retail markets in Libya using cultural and molecular techniques, and to study their antibiotics resistance profiles. A total of 139 samples from red meat, meat products, and seafood were collected from many areas in Libya. Enumeration and isolation of spp. and by normal cultural methods followed by molecular identification using molecular techniques by bacterial DNA extraction and partial sequencing of 16S rDNA. Out of 139 samples, 112 (80.6%) were contaminated with different species of based on cultural characteristics of on Baird-Parker medium, for which was detected in only 32 samples (23%). However, only six out of 18 (33.3%) isolates sent for sequencing were confirmed to be using the molecular technique. The six identified isolates of were tested for antimicrobial resistance against 24 most commonly used antibiotics. All isolates were resistant to only two antibiotics (cefotaxime and clindamycin). Among these six isolates, only one confirmed to be . Results of this study suggest that food of animal origin could be a source of with antimicrobial resistance characteristics that can be spread through the food chain, and raise the importance of these results for public health.