The aim of this study was to estimate body image perception in undergraduate students, and to investigate its associations with weight status, abdominal obesity, muscularity, gender and sport. The sample consisted of 231 Italian students (174 males and 57 females); anthropometric measurements, taken by trained technicians, were: height, weight, arm-circumference, waist and hip circumferences. BMI, WHR and Δ arm-circumference were calculated. Body image was assessed using body silhouette charts. Information about sport (currently practiced sport, starting age, and weekly hours of sport) was acquired with questionnaires. Females perceived themselves as slightly overweight, while males identified themselves as normal weight. Females had a tendency to desire to be thinner in all weight status categories; in males, normal weight subjects had a tendency to desire to be larger, while overweight wished to be thinner. Sport practice was significantly higher in males. Individuals who were overweight and did less sport were significantly more likely to have higher body dissatisfaction. The present study highlights a positive relationship between sport practice, corpulence and body image perception.