spp. are Gram-negative zoonotic bacteria transmitted to humans via various blood-sucking arthropods. Rodents have been identified as reservoir hosts of several zoonotic pathogens, including spp. In Thailand, studies of spp. in rodents from urban areas are limited; thus, a study in this area is necessary. The objectives of this study were to detect spp. in rodents in Thailand and to compare the species' distribution across different areas. In total, 70 blood samples from rodents in urban and suburban areas were tested for spp. using a conventional polymerase chain reaction that targeted the citrate synthase ( ) gene. All -positive sequences were analyzed using polymorphism in order to build a phylogenetic tree. Approximately 38% of the rodents studied contained DNA. Both (Pacific rat) and (Asian house rat) contained spp. Four species of were detected in blood samples: , , , and . In addition, eight Pacific rats contained the - complex. and - complexes were found in a specific habitat ( < 0.05). Interestingly, only seven haplotypes were identified in the sequences analyzed, and only haplotype A was found in both rodent species. Finally, a monitoring program for zoonotic infection, especially the - complex, , , and should be established, especially in high-risk areas.