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Xu, Jie; Chen, Xi; Li, Donghai; Chen, Qun; Zhou, Zhen; Hou, Dongxia; Wang, Jin; Zhang, Qipeng; Zen, Ke; Zhang, Chen-Yu
Protein & cell, 01/2015, Letnik: 6, Številka: 1Journal Article
SMALL RNA IN REVERSE TRANSCRIPTASE： TOO SMALL TO BE FOUND Significant background in no-template or no-primer RT-PCR is frequently noted. However, the underlying mechanism is still unclear. In this issue, Jie Xu and colleagues reveal that the com- mercial reverse transcriptases contain small RNAs. In AMV reverse transcriptase, they detected microRNAs derived from the virus host chick or other birds. This surprising finding at least partially explains the high background in some AMV reverse transcriptases based RT-PCR.
