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He, Jingyan; Fu, Ting; Zhang, Ling; Wanrong Gao, Lucy; Rensel, Michelle; Remage-Healey, Luke; White, Stephanie A.; Gedman, Gregory; Whitelegge, Julian; Xiao, Xinshu; Schlinger, Barney A.
Gene, 11/2022, Letnik: 843Journal Article
•In this study, we utilized the publicly available RNA-seq data generated from Illumina-based short-reads and PacBio single-molecule real-time (SMRT) long-reads to assess the zebra finch brain transcriptome.•To analyze the high-throughput RNA-seq data, we adopted a hybrid bioinformatic approach combining short and long-read pipelines.•We added 220 novel genes and 8134 transcript variants to the Ensembl annotation, and predicted a new proteome based on the refined annotation.•Using mass-spectrometry of zebra finch caudal telencephalon, we validated 18 different novel proteins.•Our results provide additional resources for future studies of zebra finches utilizing this improved bird genome annotation and proteome. The zebra finch (Taeniopygia guttata), a representative oscine songbird species, has been widely studied to investigate behavioral neuroscience, most notably the neurobiological basis of vocal learning, a rare trait shared in only a few animal groups including humans. In 2019, an updated zebra finch genome annotation (bTaeGut1_v1.p) was released from the Ensembl database and is substantially more comprehensive than the first version published in 2010. In this study, we utilized the publicly available RNA-seq data generated from Illumina-based short-reads and PacBio single-molecule real-time (SMRT) long-reads to assess the bird transcriptome. To analyze the high-throughput RNA-seq data, we adopted a hybrid bioinformatic approach combining short and long-read pipelines. From our analysis, we added 220 novel genes and 8,134 transcript variants to the Ensembl annotation, and predicted a new proteome based on the refined annotation. We further validated 18 different novel proteins by using mass-spectrometry data generated from zebra finch caudal telencephalon tissue. Our results provide additional resources for future studies of zebra finches utilizing this improved bird genome annotation and proteome.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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