The aim of this paper was to determine spatial and seasonal variation of 6 indicator polychlorinated biphenyls (PCBs) and 18 organochlorine pesticides (OCPs) in urban soils of Novi Sad, Serbia. Sixty surface soil samples were collected during both winter and summer season, from different types of locations: schools, recreational areas, residential and industrial zones. Samples were treated using the method of accelerated solvent extraction, which allows simultaneous extraction and clean up of PCBs and OCPs. Targeted compounds were analyzed by GC-μECD and confirmed by GC-MS. The total concentrations of studied PCBs varied from <LOD to 18.9 ng g−1 (mean 2.84 ng g−1 and median value of 1.21 ng g−1) in winter and from <LOD to 24.6 ng g−1 (mean 3.27 ng g−1 and median value of 1.60 ng g−1) in summer. Regarding the OCPs analyzed, dichlorodiphenyltrichloroethanes (DDTs) were only detected in both seasons. The total concentration of DDTs ranging from <LOD to 86.3 ng g−1 (mean 7.57 ng g−1 and median value of 1.87 ng g−1) in summer and from <LOD to 182 ng g−1 (mean 15.6 ng g−1 and median value of 3.15 ng g−1) in winter. There were no significant difference between seasons for studied PCBs and DDTs. Spatial distribution of PCBs and DDTs were also evaluated using Arc GIS. Additional, principle component analysis was performed to evaluate relationships between PCBs, DDTs and soil characteristics. Positive correlation was observed among p,p′-DDE, p,p′-DDD and organic matter. The total lifetime carcinogenic risk indicated that the lifetime cancer risk was acceptable. •Novel method of pressurized liquid extraction for PCBs and OCPs in soil was developed.•The results revealed that the seasonal variation of PCBs and DDTs was rather stable.•Health risk assessment suggested that Novi Sad is suitable as residential.•Profiles of DDTs indicated aged residues.•OCPs residues and PCBs in soil are not a serious public health threat in Novi Sad.