Fruit tree frost, an agricultural meteorological disaster primarily occurring in spring, severely impacts the growth and development of trees and fruit set. Therefore, exploring the temperature change rules of fruit trees during frost events is of considerable importance. This study used a T40 unmanned aerial vehicle (UAV) as the test vehicle based on the air disturbance frost prevention principle. The airflow field change pattern of the UAV was explored using the computational fluid dynamics simulation method, and the reliability of the multi-rotor UAV in frost prevention was verified using field tests. Setting the UAV height at 6.0 m above the ground and the rotor speed at 1000 r min−1, it was found that under the conditions of the inverse temperature phenomenon, the temperatures of different altitude layers will eventually converge to a stable value when the UAV down-rotating airflow is disturbed, which could be completed rapidly within 20 s. The results of the field test also showed that when the fruit trees reached the critical temperature of frost at the flowering stage, UAV intervention could have a substantial effect on the warming of the near-surface layers, whose warming amplitude was in the range of 2.5 °C–3 °C. This study provides a reliable method for mitigating frost, which serves as an important theoretical and guiding basis for improving agricultural frost disasters in China. •A multi-rotor UAV is used to test frost protection performance on fruit trees.•The temperature field characterizations are obtained.•The anti-frost effectiveness of UAV intervention is confirmed.