Paramphistomum and Fasciola are the most common parasite found in the gastrointestine of the cattle in the terai region of Nepal. The parasite resides in the gut and feed there, resulting in the nutrition deficit for the cattle. A study was conducted in Kohalpur, Banke of Nepal to study the seasonal and breed-wise prevalence of the gastrointestinal parasite in cattle. A total of 156 fecal samples were analyzed, out of which 79 samples were collected in summer and 77 samples were collected in winter. Out of total samples 108 were brought to the clinic and 48 samples were collected directly from the rumen of the cattle. Sedimentation technique for recovering the larva and egg of parasite was performed. Five slides were prepared from each sample and was observed under the microscope for eggs and larva. The result showed that the prevalence was 36.71% in the summer and it was 16.88% in the winter. The statistical relationship showed that the prevalence gastrointestinal parasite in cattle was significantly (P<0.05) higher in the summer season. Likewise, the prevalence was 11.86% in the Jersey breed and 36.08% in the Jersey cross breed. And the statistical relationship also showed that the gastrointestinal prevalence in Jersey cross breed was significantly (P<0.05) higher than Jersey breed.