•Water quality indices are useful for indicating total effect of ecological factors.•The results can help local people in improving water quality of Al-Gharraf River.•Al-Gharraf water is unpotable (67.3 WQI) due to natural and anthropogenic factors.•Al-Gharraf River water is poor for aquatic life but fair for irrigation. The Water Quality Index has been developed mathematically to evaluate the water quality of Al-Gharraf River, the main branch of the Tigris River in the south of Iraq. Water samples were collected monthly from five sampling stations during 2015–2016, and 11 parameters were analyzed: biological oxygen demand, total dissolved solids, the concentration of hydrogen ions, dissolved oxygen, turbidity, phosphates, nitrates, chlorides, as well as turbidity, total hardness, electrical conductivity and alkalinity. The index classified the river water, without including turbidity as a parameter, as good for drinking at the first station, poor at stations 2, 3, 4 and very poor at station 5. When turbidity was included, the index classified the river water as unsuitable for drinking purposes in the entire river. The study highlights the importance of applying the water quality indices which indicate the total effect of the ecological factors on surface water quality and which give a simple interpretation of the monitoring data to help local people in improving water quality.