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Denisov, Eduard; Damoc, Eugen; Makarov, Alexander
International journal of mass spectrometry, August 2021, 2021-08-00, Letnik: 466Journal Article
Experiments on a modified Orbitrap Exploris instrument have shown that even in a compact instrument it is possible to achieve a resolving power in excess of 2,000,000 when appropriate tolerance and tuning requirements are met. Such levels are achieved for a 4-s detection time. A new Orbitrap design with improved pumping allowed, for the first time, isotopic resolution of intact monoclonal antibodies even when using standard nitrogen supply in the Ion Routing Multipole. Longer transients further improve signal-to-noise ratios, approaching levels needed for single-charge resolution in charge detection mass spectrometry. Further steps for improvement of instrumentation are discussed. Display omitted •Compact high-field Orbitrap analyzer is employed on Orbitrap Exploris platform.•Resolving power in excess of 2,000,000 is demonstrated for Orbitrap MS.•Examples of applications include intact antibody analysis with isotopic resolution and detection of individual peptide ions.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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