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Katsman, Efrat; Orlanski, Shari; Martignano, Filippo; Fox-Fisher, Ilana; Shemer, Ruth; Dor, Yuval; Zick, Aviad; Eden, Amir; Petrini, Iacopo; Conticello, Silvestro G.; Berman, Benjamin P.
Genome Biology, 07/2022, Letnik: 23, Številka: 1Journal Article
Abstract The Oxford Nanopore (ONT) platform provides portable and rapid genome sequencing, and its ability to natively profile DNA methylation without complex sample processing is attractive for point-of-care real-time sequencing. We recently demonstrated ONT shallow whole-genome sequencing to detect copy number alterations (CNAs) from the circulating tumor DNA (ctDNA) of cancer patients. Here, we show that cell type and cancer-specific methylation changes can also be detected, as well as cancer-associated fragmentation signatures. This feasibility study suggests that ONT shallow WGS could be a powerful tool for liquid biopsy. Graphical Abstract
