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Wang, Li; Kaya, Hilal Betul; Zhang, Ning; Rai, Rhitu; Willmann, Matthew R; Carpenter, Sara C D; Read, Andrew C; Martin, Federico; Fei, Zhangjun; Leach, Jan E; Martin, Gregory B; Bogdanove, Adam J
Frontiers in genome editing, 03/2021, Letnik: 3Journal Article
Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in , rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Vir: Osebne bibliografije
in: SICRIS
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