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Goudal, Adeline; Laude, Adrien; Valot, Stéphane; Desoubeaux, Guillaume; Argy, Nicolas; Nourrisson, Céline; Pomares, Christelle; Machouart, Marie; Le Govic, Yohann; Dalle, Frédéric; Botterel, Françoise; Bourgeois, Nathalie; Cateau, Estelle; Leterrier, Marion; Lavergne, Rose-Anne; Beser, Jessica; Le Pape, Patrice; Morio, Florent
Diagnostic microbiology and infectious disease, January 2019, 2019-Jan, 2019-01-00, 20190101, 2019-01, Letnik: 93, Številka: 1Journal Article
Microscopy is the gold standard for the diagnosis of gastrointestinal parasites but is time-consuming and dependent on operator skills. Rapid diagnostic tests represent alternative methods but most evaluations have been conducted on a limited number of samples preventing their implementation in the clinical setting. We evaluated a new CE-IVD marked immunochromatographic assay (Crypto/Giardia K-SeT®, Coris Bioconcept) for the detection of G. intestinalis and Cryptosporidium spp. in 2 phases (retrospective and prospective) on a set of 482 stool samples including rare Cryptosporidium species. Besides G. intestinalis, this test could represent a rapid and reliable alternative to the modified Ziehl-Neelsen staining for the diagnosis of cryptosporidiosis (sensitivity/specificity were 89.2%/99.3% and 86.7%/100% for G. intestinalis and Cryptosporidium resp.), reducing diagnostic delays. Such strategy would also be time-saving by avoiding wet mount microscopy and concentrations steps, being particularly appropriate for laboratories having little expertise in microscopy or not able to implement molecular diagnostic methods. •Microscopy is a standard for the diagnosis of gastrointestinal parasitic infections.•Rapid diagnostic tests (RDTs) offer a shorter hands-on time.•We evaluated a new RDT targeting G. intestinalis and Cryptosporidium spp.•The new RDT could represent a rapid and reliable alternative to microscopy.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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