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Trbojević-Akmačić, Irena; Vučković, Frano; Pribić, Tea; Vilaj, Marija; Černigoj, Urh; Vidič, Jana; Šimunović, Jelena; Kępka, Agnieszka; Kolčić, Ivana; Klarić, Lucija; Novokmet, Mislav; Pučić-Baković, Maja; Rapp, Erdmann; Štrancar, Aleš; Polašek, Ozren; Wilson, James F; Lauc, Gordan
Communications biology, 03/2023, Letnik: 6, Številka: 1Journal Article
Human plasma transferrin (Tf) N-glycosylation has been mostly studied as a marker for congenital disorders of glycosylation, alcohol abuse, and hepatocellular carcinoma. However, inter-individual variability of Tf N-glycosylation is not known, mainly due to technical limitations of Tf isolation in large-scale studies. Here, we present a highly specific robust high-throughput approach for Tf purification from human blood plasma and detailed characterization of Tf N-glycosylation on the level of released glycans by ultra-high-performance liquid chromatography based on hydrophilic interactions and fluorescence detection (HILIC-UHPLC-FLD), exoglycosidase sequencing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). We perform a large-scale comparative study of Tf and immunoglobulin G (IgG) N-glycosylation analysis in two human populations and demonstrate that Tf N-glycosylation is associated with age and sex, along with multiple biochemical and physiological traits. Observed association patterns differ compared to the IgG N-glycome corroborating tissue-specific N-glycosylation and specific N-glycans' role in their distinct physiological functions.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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