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  • Metabolite Profiling of Gar...
    Krasteva, Gergana; Berkov, Strahil; Pavlov, Atanas; Georgiev, Vasil

    Molecules (Basel, Switzerland), 12/2022, Letnik: 27, Številka: 24
    Journal Article

    Ellis is an aromatic and medicinal plant of high economic value. Much research has focused on the phytochemistry and biological activities of fruit extracts; however, the potential of the plant in vitro cultures used as mass production systems of valuable secondary metabolites has been understudied. This paper presents data on metabolite profiling (GC/MS and HPLC), antioxidant activities (DPPH, TEAC, FRAP, and CUPRAC), and SSR profiles of plant leaves and in vitro cultures with different levels of differentiation (shoots, callus, and cell suspension). The data show strong correlations (r = 0.9777 to r = 0.9908) between antioxidant activity and the concentrations of chlorogenic acid, salicylic acid, rutin, and hesperidin. Eleven co-dominant microsatellite simple sequence repeats (SSRs) markers were used to evaluate genetic variations (average PIC = 0.738 ± 0.153). All of the investigated in vitro cultures showed high genetic variabilities (average Na = 5.636 ± 2.157, average Ne = 3.0 ± 1.095). This is the first report on a study on metabolite profiles, antioxidant activities, and genetic variations of in vitro cultures with different levels of differentiation.