E-viri
Recenzirano
Odprti dostop
-
Kaustio, Meri, MSc; Haapaniemi, Emma, MD, PhD; Göös, Helka, MSc; Hautala, Timo, MD, PhD; Park, Giljun, PhD; Syrjänen, Jaana, MD, PhD; Einarsdottir, Elisabet, PhD; Sahu, Biswajyoti, PhD; Kilpinen, Sanna, MD, PhD; Rounioja, Samuli, MD, PhD; Fogarty, Christopher L., MSc; Glumoff, Virpi, PhD; Kulmala, Petri, MD, PhD; Katayama, Shintaro, PhD; Tamene, Fitsum, MSc; Trotta, Luca, MSc; Morgunova, Ekaterina, PhD; Krjutškov, Kaarel, PhD; Nurmi, Katariina, PhD; Eklund, Kari, MD, PhD; Lagerstedt, Anssi, MD, PhD; Helminen, Merja, MD, PhD; Martelius, Timi, MD, PhD; Mustjoki, Satu, MD, PhD; Taipale, Jussi, PhD; Saarela, Janna, MD, PhD; Kere, Juha, MD, PhD; Varjosalo, Markku, PhD; Seppänen, Mikko, MD, PhD
Journal of allergy and clinical immunology, 09/2017, Letnik: 140, Številka: 3Journal Article
Background The nuclear factor κ light-chain enhancer of activated B cells (NF-κB) signaling pathway is a key regulator of immune responses. Accordingly, mutations in several NF-κB pathway genes cause immunodeficiency. Objective We sought to identify the cause of disease in 3 unrelated Finnish kindreds with variable symptoms of immunodeficiency and autoinflammation. Methods We applied genetic linkage analysis and next-generation sequencing and functional analyses of NFKB1 and its mutated alleles. Results In all affected subjects we detected novel heterozygous variants in NFKB1 , encoding for p50/p105. Symptoms in variant carriers differed depending on the mutation. Patients harboring a p.I553M variant presented with antibody deficiency, infection susceptibility, and multiorgan autoimmunity. Patients with a p.H67R substitution had antibody deficiency and experienced autoinflammatory episodes, including aphthae, gastrointestinal disease, febrile attacks, and small-vessel vasculitis characteristic of Behçet disease. Patients with a p.R157X stop-gain experienced hyperinflammatory responses to surgery and showed enhanced inflammasome activation. In functional analyses the p.R157X variant caused proteasome-dependent degradation of both the truncated and wild-type proteins, leading to a dramatic loss of p50/p105. The p.H67R variant reduced nuclear entry of p50 and showed decreased transcriptional activity in luciferase reporter assays. The p.I553M mutation in turn showed no change in p50 function but exhibited reduced p105 phosphorylation and stability. Affinity purification mass spectrometry also demonstrated that both missense variants led to altered protein-protein interactions. Conclusion Our findings broaden the scope of phenotypes caused by mutations in NFKB1 and suggest that a subset of autoinflammatory diseases, such as Behçet disease, can be caused by rare monogenic variants in genes of the NF-κB pathway.
Avtor
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.