This study examined the influence of birth date, salivary testosterone sT concentration, sexual maturity status, and general strength on the selection process of an elite Brazilian soccer club during a 12-month period, which was a 2 phase of a 24-month selection process. The stature, body mass, sT, sexual maturity status Tanner scale, and hand grip strength were assessed for 143 players during 2 weeks. From these 143 players, 100 players were dismissed DIS and 43 players were selected to integrate the club's under-14 squad. Following 1-year training period, the under-14 team was assembled with 9 players designated as starters STA, and then, comparisons were conducted taking into account a group of non-starters (selected; SEL = 34 players) and STA (n = 09). The DIS, SEL, STA players, and reference population, were compared for birth distribution. A greater proportion of players was born in the first trimester in the STA 75.0%, SEL 57.1% and DIS 50.0% groups compared to the reference population 25.8%. One-way ANOVA showed a higher sT for STA group 516.0 ± 129.9 pmol·L , compared to SEL 415.5 ± 117.9 pmol·L and DIS groups 390.9 ± 84.9 pmol·L ; p = 0.003), and Kruskall-Wallis test showed a higher gonadal development for STA compared to DIS p = 0.001. The current findings suggest a strong influence of birth date and biological maturation on young soccer players selection process. Soccer coaches should consider these influences when making decisions about player selection of elite youth players.