The NeoPRINT Survey was designed to assess premedication practices throughout UK NHS Trusts for both neonatal endotracheal intubation and less invasive surfactant administration (LISA). An online survey consisting of multiple choice and open answer questions covering preferences of premedication for endotracheal intubation and LISA was distributed over a 67-day period. Responses were then analysed using STATA IC 16.0. Online survey distributed to all UK Neonatal Units (NNUs). The survey evaluated premedication practices for endotracheal intubation and LISA in neonates requiring these procedures. The use of different premedication categories as well as individual medications within each category was analysed to create a picture of typical clinical practice across the UK. The response rate for the survey was 40.8 % (78/191). Premedication was used in all hospitals for endotracheal intubation but overall, 50 % (39/78) of the units that have responded, use premedications for LISA. Individual clinician preference had an impact on premedication practices within each NNU. The wide variability on first-line premedication for endotracheal intubation noted in this survey could be overcome using best available evidence through consensus guidance driven by organisations such as British Association of Perinatal |Medicine (BAPM). Secondly, the divisive view around LISA premedication practices noted in this survey requires an answer through a randomised controlled trial. •Premedication was used in all UK neonatal units for endotracheal intubation.•Seven different pre-medication combinations were used for analgesia/sedation prior to endotracheal intubation.•Clinicians’ opinion regarding premedication for LISA was divisive because of lack of good quality evidence.