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Ma, Jiyan
PLOS pathogens, 04/2012, Letnik: 8, Številka: 4Journal Article
The simultaneous propagation of protease-resistant PrPSc and prion infectivity in sPMCA provides strong evidence supporting the prion hypothesis 5. Because crude brain homogenate is used in sPMCA for prion propagation, it is difficult to conclusively establish a causal relationship between PrPSc and prion disease. More importantly, the infectivity of prions generated via sPMCA has been demonstrated by causing bona fide prion disease in wild-type animals 9, 10, 11. Because bacterially expressed recPrP does not contain any eukaryotic genetic informational molecules, generating prion infectivity with recPrP 10-12 is generally accepted as the most stringent proof of prion hypothesis.
