All nextGen sequencing technologies require library construction as a first step where platform-specific adaptors are ligated to target DNA fragments. Pacific Biosciences RS technology requires 5-10ug of DNA for large insert (∼10kb) library construction. However, under circumstances such as when performing long-range PCR on DNA regions containing repetitive sequences or other difficult to amplify targets, only limited product is available. Cloning, which poses sequence stability problems due to the repetitive nature of the insert, is the only other option for generating sufficient DNA under these circumstances. Here, we demonstrate the direct sequencing of a 10kb linear DNA fragment starting with as little as 150ng input DNA using PacBio version 2 chemistry. These experiments were performed before PacBio released their magbead station upgrade. The magbead station reduces the amount of input DNA required for polymerase binding and this technical advance will clearly reduce the amount of input DNA required for directly sequencing linear DNA templates over the requirements of our initial experiment.