This study investigated the relationship between singing ability and spatial ability in preschoolers between the ages of 4 and 6 years old. The purpose was to examine cognitive ability associated with singing ability, in considering how to support singing in the field of early childhood education. We measured verbal ability and age as a control variable. Singing ability was measured using the "AIRS Test Battery of Singing Skills (ATBSS) for Children-Revised" (Ogura & Adachi, 2018). Spatial ability and verbal ability were measured using the WPPSI-III Block Design and Vocabulary. The results of multiple regression analysis showed that preschoolers with higher spatial ability had higher singing ability. No association between singing ability against verbal ability and age was identified. We concluded that the relationship of spatial and singing abilities was confirmed because both abilities were associated with perception and motor integration. Future intervention studies should be conducted in early childhood education settings.