Abstract Background: Team-based learning (TBL) is a student-centered approach which has been used in health professions education and has demonstrated effectiveness in promoting students' learning, engagement, and application of knowledge. This study aimed to assess undergraduate nursing students' perception about TBL as an instructional strategy at Nile University. Methods: A descriptive cross-sectional study was carried out among undergraduate nursing students at Nile University in Sudan. A structured questionnaire was filled out by 43 students out of 48 total students in cardiothoracic module, where 5 students refused to participate. Data were analyzed by descriptive and inferential statistics using the SPSS version 16. Results: The findings showed that nursing students had high level of agreement with TBL in all domains of study, the higher score was noted in learning and engagement and motivation (86.80% and 87.44%, respectively). They demonstrated high positive perception on the usage of TBL in nursing education, P-value = 0.00. Conclusion: The study concluded that nursing students have a positive perception about TBL method and that it improved students' learning behaviors; the more effect was in students' learning and communication skills in speaking English and transferring knowledge to others.