Provider: - Institution: - Data provided by Europeana Collections- U radu je istražena mogućnost primjene titanatnih nanocjevčica kao čvrstog nosača za imobilizaciju katalizatora u elektrokemijskim (amperometrijskim) senzorima. Titanatne nanocjevčice površinski su modificirane (3-aminopropil)trimetoksisilanom (APTMS), pri čemu je istražen utjecaj reakcijskih uvjeta na strukturu siloksanskog sloja nastalog na površini titanatnih nanocjevčica. Površinske amino-skupine titanatnih nanocjevčica modificiranih s APTMS iskorištene se kao reaktivna mjesta za kovalentnu imobilizaciju redoks-enzima peroksidaze, odn. kovalentno vezanje katalitički aktivnog N-saliciliden-n-propilaminskog kompleksa željeza(III) na površinu titanatnih nanocjevčica. Titanatnim nanocjevčicama modificiranim peroksidazom, odn. N-saliciliden-n-propilaminskim kompleksom željeza(III) istražena su elektrokemijska i elektrokatalitička svojstva. Ustanovljeno je da peroksidaza nakon imobilizacije na površinu titanatnih nanocjevčica zadržava katalitičku aktivnost te da molekule imobilizirane peroksidaze mogu sudjelovati u izravnom prijenosu elektrona između redoks-centra enzima i elektrode, odn. vodljive matrice u koju su nanocjevčice ugrađene. Titanatne nanocjevčice s imobiliziranom peroksidazom s uspjehom su uporabljene za izradu amperometrijskog biosenzora za detekciju vodikova peroksida i dvoenzimskog biosenzora za određivanje glukoze.- In this thesis, the possibility of application of titanate nanotubes as a solid substrate for immobilization of catalysts in electrochemical (amperometric) sensors was studied. Titanate nanotubes were surface modified with (3-aminopropyl)trimethoxysilane (APTMS) and the influence of reaction conditions on the structure of siloxane layer formed on the surface of titanate nanotubes was studied. Surface amino-groups of titanate nanotubes modified with APTMS were exploited as a reactive sites for covalent immobilization of the redox enzyme peroxidase and for covalent bonding of N-salicyliden-n-propylamino complex of iron(III) on the surface of titanate nanotubes. Titanatne nanototubes modified with peroxidase or with N-salicyliden-n-propylamino complex of iron(III) were embedded into the conductive graphite composite and its electrocatalytic properties were examined. It was found that after immobilization on the surface of titanate nanotubes, peroxidase retains its catalytic activity and that the molecules of immobilized peroxidase can effectively participate in direct electron transfer between the enzyme redox center and electrode or the conductive matrix in which the nanotubes were embedded. Titanate nanotubes with immobilized peroxidase were successfully employed in the construction of amperometric hydrogen peroxide biosensors of the third generation and in the construction of the bi-enzyme biosensor for glucose detection.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana