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  • Desmosome assembly and cell-cell adhesion are membrane raft-dependent processes
    Resnik, Nataša ...
    The aim of our study was to investigate the association of desmosomal proteinswith cholesterol-enriched membrane domains, commonly called membrane rafts, and the influence of cholesterol on desmosome ... assembly in epithelial MDCK cells (clone MDc-2). Biochemical analysis proved an association of desmosomal cadherin desmocollin 2 (Dsc2) in cholesterol-enriched fractions that contain membrane raft markers caveolin-1 and flotillin-1 and the novel raft marker ostreolysin. Cold detergent extraction of biotinylated plasma membranes revealed that ~ 60% of Dsc2 associates with membrane rafts while theremainder is present in non-raft and cholesterol-poor membranes. The results of immunofluorescence microscopy confirmed colocalization of Dsc2 and ostreolysin. Partial depletion of cholesterol with methyl-beta-cyclodextrin disturbs desmosome assembly, as revealed by sequential recordings of live cells. Moreover, cholesterol depletion significantly reduces the strength of cell-cell junctions and partially releases Dsc2 from membrane rafts. Our dataindicate that a pool of Dsc2 is associated with membrane rafts; particularly with the ostreolysin type of membrane raft and that intact membrane rafts are necessary for desmosome assembly. Taken together, these data suggest cholesterol as a potential regulator that promotes desmosome assembly.
    Source: The Journal of biological chemistry. - ISSN 0021-9258 (Vol. 286, issue 2, 2011, str. 1499-1507)
    Type of material - article, component part
    Publish date - 2011
    Language - english
    COBISS.SI-ID - 27616729
    DOI

source: The Journal of biological chemistry. - ISSN 0021-9258 (Vol. 286, issue 2, 2011, str. 1499-1507)
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