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Desmosome assembly and cell-cell adhesion are membrane raft-dependent processesResnik, Nataša ...The aim of our study was to investigate the association of desmosomal proteinswith cholesterol-enriched membrane domains, commonly called membrane rafts, and the influence of cholesterol on desmosome ... assembly in epithelial MDCK cells (clone MDc-2). Biochemical analysis proved an association of desmosomal cadherin desmocollin 2 (Dsc2) in cholesterol-enriched fractions that contain membrane raft markers caveolin-1 and flotillin-1 and the novel raft marker ostreolysin. Cold detergent extraction of biotinylated plasma membranes revealed that ~ 60% of Dsc2 associates with membrane rafts while theremainder is present in non-raft and cholesterol-poor membranes. The results of immunofluorescence microscopy confirmed colocalization of Dsc2 and ostreolysin. Partial depletion of cholesterol with methyl-beta-cyclodextrin disturbs desmosome assembly, as revealed by sequential recordings of live cells. Moreover, cholesterol depletion significantly reduces the strength of cell-cell junctions and partially releases Dsc2 from membrane rafts. Our dataindicate that a pool of Dsc2 is associated with membrane rafts; particularly with the ostreolysin type of membrane raft and that intact membrane rafts are necessary for desmosome assembly. Taken together, these data suggest cholesterol as a potential regulator that promotes desmosome assembly.Source: The Journal of biological chemistry. - ISSN 0021-9258 (Vol. 286, issue 2, 2011, str. 1499-1507)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 27616729
Author
Resnik, Nataša |
Sepčić, Kristina |
Plemenitaš, Ana, 1952- |
Windoffer, Reinhard |
Leube, Rudolf |
Veranič, Peter
Topics
Desmosomes |
Cell Communication |
Cell Adhesion Molecules |
Cholesterol |
Cadherins |
Intracellular Membranes |
Microscopy, Fluorescence |
Cells, Cultured |
Blotting, Western |
Holesterol |
Celična komunikacija |
Celična adhezija, molekule |
Dezmosomi |
Kadherini |
Znotrajcelične membrane |
Blot, Western |
Celične kulture |
Mikroskopija fluorescenčna
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Resnik, Nataša | 28393 |
Sepčić, Kristina | 15328 |
Plemenitaš, Ana, 1952- | 06777 |
Windoffer, Reinhard | |
Leube, Rudolf | |
Veranič, Peter | 08279 |
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