The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.); Vpliv postopkov shranjevanja in ekstrakcije celokupne DNA na rezultate molekularne analize osmerozobega smrekovega lubadarja (Ips typographus L.)

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The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.) = Vpliv postopkov shranjevanja in ekstrakcije celokupne DNA na rezultate molekularne analize osmerozobega smrekovega lubadarja (Ips typographus L.)

Devetak, Zina ...

Za uspeh molekularne identifikacije podlubnikov je ključna zadostna količina kakovostnega ekstrakta DNA. Pri raziskavi vrste smo se osredotočili na vpliv različnih postopkov shranjevanja osebkov Ips ... typographus(L.) in različnih protokolov ekstrakcije DNA na količino in kakovost DNA, namenjene za uporabo v molekularni diagnostiki. Odrasle hrošče smo zamrznili pri -20 °C na dva načina, v etanolu in na suho. Ekstrakcije smo opravili po štirih različnih postopkih. Primerjali smo količino pridobljene DNA. Kakovost ekstrahirane DNA smo ocenili s polimerazno verižno reakcijo in sekvenciranjem po Sangerju. Način shranjevanja ni statistično značilno vplival na količino pridobljene DNA. DNA višje kakovosti smo pridobili iz hroščev, ki so bili zamrznjeni v etanolu. Le dva postopka ekstrakcije DNA sta dala DNA, uporabno za sekvenciranje. V količini pridobljene DNA se pri sekvenciranju uspešna postopka nista pomembno razlikovala. Določili smo optimalno kombinacijo postopkov shranjevanja v etanolu pri -20 °C in ekstrakcije celokupne DNA z ekstrakcijskim kitom Nucleospin Insect DNA proizvajalca Macherey Nagel, ki omogoča časovno učinkovito identifikacijo.

Source: Acta Silvae et Ligni. - ISSN 2335-3112 ([Št.] 132, 2023, str. 29-38)

Type of material - article, component part

Publish date - 2023

Language - english

COBISS.SI-ID - 180304131

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DOI

Author	Devetak, Zina ...
Title	The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.) = Vpliv postopkov shranjevanja in ekstrakcije celokupne DNA na rezultate molekularne analize osmerozobega smrekovega lubadarja (Ips typographus L.)
Publication date	2023-12-31
COBISS.SI-ID	180304131
Publication version in repository	Publisher's version
Publication licence	Creative Commons Attribution-ShareAlike 4.0 International
Embargo	Immediate publication for public

Project(s) from which the publication was funded

Title	Acronym	Project ID	Funder
Gozdna biologija, ekologija in tehnologija		P4-0107-2020	Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Administration of the Republic of Slovenia for Food Safety, VeterinarySector and Plant Protection		C2337-21-000016
Administra-tion of the Republic of Slovenia for Food Safety, VeterinarySector and Plant Protection		C2337-22-000020
Javna gozdarska služba	JGS	JGS	Ministrstvo za kmetijstvo, gozdarstvo in prehrano

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source: Acta Silvae et Ligni. - ISSN 2335-3112 ([Št.] 132, 2023, str. 29-38)

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Year	JCR	SNIP	JCR	SNIP	JCR	SNIP	JCR	SNIP

Links to authors' personal bibliographies	Links to information on researchers in the SICRIS system
Devetak, Zina	32514
Kavčič, Andreja, biologinja	33222
De Groot, Maarten, entomolog	29092
Piškur, Barbara, 1979-	25448

Source: Personal bibliographies and: SICRIS

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