Mehanizem transporta glukoze v osmotskem šoku v inzulindko rezistentnih 3T3-L1 adipocitih = Mechanism of osmotic shock-induced glucose transport in insulin resistant 3T3-L1 adipocytes : doctoral dissertation
Janež, Andrej, 1971-
Osmotic shock treatment of 3T3-L1 adipocytes causes an increase in glucose transport activity and translocation of GLUT-4 protein similar to that elicited by insulin treatment. Insulin stimulation of ...GLUT-4 translocation andglucose transport activity was completely inhibited by wortmannin, however,activation by osmotic shock was only partialy blocked. Additionally, we have found that the newly identified insulin rcceptor substrate (IRS) Gab-1(Grb2-associated binder-1) is tyrosine phosphorylated following sorbitol stimulation. Treatment of cells with the tyrosine kinase inhibitor genestin inhibited osmotic shockstimulated Gab-1 phosphorylation, as well as shock-induced glucose transport. rurthennore, pretreatment with the selective Src family kinase inhibitor PP2 completely inhibited the ability of sorbitol treatment to cause tyrosine phosphorylation of Gab-1. We have also shown that microinjection of anti-Gab-1 antibody inhibits osmotic shock-induced GLUT-4 translocation. Furthermore, phosphorylated Gab-1 binds and activates phosphatidylinositol 3-kinase (PI 3-kinase) in response to osmotic shock. The PI 3-kinase activity associated with Gab-1 was 82% of that associated with antiphosphotyrosine, indicating that Gab-I is the major site for PI 3-kinase recruitment following osmotic shock stimulation. (Abstract truncated at 2000 characters)
Type of material - dissertation
Publication and manufacture - Ljubljana : [A. Janež], 2000
doktorske disertacije JANEŽ Andrej Mechanisms IN: 020001413
available - outside loan, loan period: 1 months
MF, Central Medical Library, Lj.
doktorske disertacije JANEŽ Andrej Mechanisms IN: 020001414
available - outside loan, loan period: 1 months
Shelf entry
Adding material to shelf was successful.
Adding material to shelf failed.
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
