Vpliv encimskega ekstrakta riževe semenske ovojnice na rast, razvoj in preživetje mezenhimskih matičnih celic, izoliranih iz maščobnega tkiva podgane = Effect of enzymatic extract from rice bran on growth, differentiation and survival of rat adipose tissue-derived mesenchymal stem cells : masters's thesis : Master's Study of Laboratory biomedicine
Škof, Maja, biokemičarka
Enzymatic extract of rice bran (EERB) is water-soluble extract that exerts strong antioxidant activity. It is obtained after enzyme hydrolysis of rice bran with endoproteases. It contains a mixture ...of water-soluble substances, rich in antioxidants (γ-oryzanol, vitamin E) and consists mainly of peptides, polysaccharides and polyunsaturated fatty acids. Adipose tissue-derived mesenchymal stem cells (ADSC) are multipotent stem cells, which are a prominent source in regenerative medicine. Because of their relatively easy isolation and broad differentiation potential, many studies are focusing on different tissue regeneration with ADSC. However, many difficulties in their growth and differentiation still exist. This Master’s thesis is a preliminary study where we evaluated the effect of EERB on different characteristics of ADSC. We researched how EERB as a cell culture medium influences proliferation of ADSC, their differentiation to neurospheres and neurons and if EERB as an antioxidant protects ADSC against oxidative stress, induces by cumene hydroperoxide (CH). EERB as a cell culture medium supplement has shown to be beneficial for growth of ADSC. In adequate concentrations EERB promotes cell growth, as we have shown with MTS assay and cell counting experiments. Also, EERB does influence ADSC gene expression, as we researched for SOX2 and eEF. In regenerative medicine, ADSC are of great interest for differentiation to neurons, though the process is arduous and strongly depended on environment and cell conditions. EERB could be an important factor in more efficient differentiation because of its antioxidative features though more research should be done on this aspect. Additionally, we showed that EERB protects ADSC against oxidative stress, that was induced by CH. However, more research on this topic is needed. The results of MTS assay and cell counting showed that EERB does protect against oxidative stress, but LDH cytotoxicity assay was showing stronger cytotoxicity when cells were incubated with CH and EERB. We can conclude that EERB is indeed a promising new factor for cells proliferation and its influence should be considered in future research.
Vrsta gradiva - magistrsko delo
; neleposlovje za odrasle
Založništvo in izdelava - Ljubljana : [M. Škof], 2024
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